Methods

Methods and analysis scope

Review the methods, reported metrics, and interpretation limits used across LamiOmicsDB tools and data views.

Taxonomy and source ingestion

Species dossiers integrate accepted names, aliases, omics-status metadata, sequence records, organelle GenBank reports, pathway source tables and imported phylogenies. Public accession fields are shown only when present in the release.

Sequence similarity

The current web tool uses a bounded local Smith–Waterman implementation with a k-mer prefilter. It is not BLAST+ or DIAMOND. Reports include identity, query coverage, subject coverage, alignment length, mismatches, gaps and the implementation-specific score.

Open similarity search

Primer candidates

The tool applies Wallace melting temperature, GC, homopolymer, self-complementarity and product-size filters. It is not Primer3 and does not check genome-wide specificity; candidates require independent validation.

Open primer design

Pathway candidates

Family-level source counts and browsable loci are reported separately. Similarity, HMM, gene-tree, motif and cluster counters originate from different pipeline stages and are not interchangeable.

Open pathway atlas

Phylogenies

Tree pages provide interactive lineage browsing and downloadable Newick files. Use terminal labels to open linked species records and internal nodes to inspect clade structure.

Open tree layers