Methods, boundaries and provenance

Pathway evidence language

Definitions used across the atlas, family, species, candidate and expression pages. Evidence states describe the released database records and do not replace experimental validation.

Status language

Do not conflate evidence states

◆

Stable locus evidence

A concrete, species-linked candidate identifier is available in the public pathway package. It is still a computational candidate, not a validated biochemical function.

□ᴿ

Reference-only family summary

A family-level assignment count or reference-library module is available, but no individual stable candidate ID is asserted.

▨

ND — Not detected

A candidate-to-expression mapping exists, but no valid quantitative value was detected in the released expression matrix. ND is distinct from numeric zero.

⊘

Unavailable

The required data are not available in this release. Unavailable never means biological absence.

Evidence modes

How candidate and family records are represented

Gene-tree evidenceCandidate identifiers from the bundled gene-tree workflow, with BLAST/HMM evidence fields retained when available. Candidate IDs
Motif-screen evidenceCandidate identifiers screened with motif proxies. Motif presence does not establish substrate or enzyme activity. Proxy evidence
Family-count evidenceSpecies-level assignment counts without individual candidate identifiers. The UI retains counts without fabricating locus IDs. Reference-only
Local 100-kb clustering proxyA coordinate-based local clustering summary. It is not full cross-species synteny. Structural proxy

Validation status

Evidence layers in the bundled release

Completed

✓
gene tree

Built FastTree trees for PAL, LAMT, and NCS from Lamiales/Lamiaceae and internal controls.

✓
domain proxy

Evaluated motif proxies for TPS, CYP76, BAHD, and RAS using local candidate protein sequences.

✓
local cluster proxy

Computed 100-kb local clustering/tandem-like proxy for key families in top Lamiales species with local GFF annotations.

✓
plantsme five pathways

PlantSME full-mode identification of alkaloid, flavonoid, phenylpropanoid, steroid and terpenoid enzyme-family candidates using genome-derived proteins or transcriptome-derived substitutes with explicit provenance.

Partial

◐
synteny

MCScanX/jcvi were not available locally; completed coordinate-based local clustering proxy instead of full cross-species synteny.

Not available locally

⊘
expression

No clear local expression atlas / RNA quantification tables were found in the repository.

⊘
metabolome

No clear local metabolomics tables were found in the repository.

Expression & ND

Quantitative display rules

Species-level transcriptome matrix summaries are shown independently. Candidate IDs are not joined to expression IDs because annotation versions do not map directly.

  • Measured values use a numeric legend and a diverging row z-score scale centred at zero.
  • ND cells use an independent hatch pattern and never occupy the low end of the continuous scale.
  • An all-ND matrix remains visible with an explicit warning; it is not converted into “no expression data”.
  • Unmapped candidate IDs remain separate from mapped-but-ND identifiers.

Re-identification provenance

Patched-library source profiles

The following provenance rows are included in the release and displayed without inferring missing values.

SpeciesValid proteinsAccepted modulesAssignmentsLibraryMode
Agastache foeniculum34,056913,53520260604-patchedfull
Agastache rugosa26,430903,82020260604-patchedfull
Ajuga decumbens36,055925,91620260604-patchedfull
Ajuga reptans36,601923,81420260604-patchedfull
Ballota pseudodictamnus44,190904,06720260604-patchedfull
Callicarpa americana62,993909,72620260604-patchedfull
Callicarpa nudiflora31,266904,67220260604-patchedfull
Clerodendrum bungei41,434923,88920260604-patchedfull
Clinopodium barosmum41,864916,19020260604-patchedfull
Clinopodium gracile40,083914,74920260604-patchedfull
Collinsonia canadensis49,336924,60020260604-patchedfull
Congea tomentosa41,401903,93420260604-patchedfull
Cornutia pyramidata73,850995,02420260604-patchedfull
Dracocephalum rupestre35,602895,36620260604-patchedfull
Drepanocaryum sewerzowii26,957933,36720260604-patchedfull
Elsholtzia splendens55,748888,81020260604-patchedfull
Glechoma hederacea45,148944,52320260604-patchedfull
Glechoma longituba34,930925,62020260604-patchedfull
Gmelina philippensis37,915933,71120260604-patchedfull
Holmskioldia sanguinea42,679944,11820260604-patchedfull
Hyptis suaveolens40,037934,25220260604-patchedfull
Hyssopus officinalis50,669897,61920260604-patchedfull
Isodon lophanthoides35,863895,06520260604-patchedfull
Isodon serra27,867914,39720260604-patchedfull
Lamium album39,532933,86120260604-patchedfull
Lavandula angustifolia44,101904,27020260604-patchedfull
Leonotis leonurus34,946893,45920260604-patchedfull
Leonurus cardiaca32,032883,48820260604-patchedfull
Leonurus japonicus28,652913,97420260604-patchedfull
Leonurus sibiricus25,733903,70020260604-patchedfull
Lycopus americanus40,879943,74520260604-patchedfull
Marmoritis complanata28,336924,15820260604-patchedfull
Marrubium vulgare33,432913,57220260604-patchedfull
Melissa officinalis32,458923,56720260604-patchedfull
Mentha longifolia20,031893,11020260604-patchedfull
Mentha spicata40,191924,09120260604-patchedfull
Mentha x piperita43,736924,58420260604-patchedfull
Monarda didyma34,278903,86020260604-patchedfull
Mosla chinensis32,297894,71120260604-patchedfull
Mosla soochowensis34,133874,64320260604-patchedfull
Nepeta cataria78,8769311,51820260604-patchedfull
Nepeta mussinii42,874866,58120260604-patchedfull
Ocimum basilicum78,9909313,07320260604-patchedfull
Ocimum tenuiflorum36,427925,45720260604-patchedfull
Origanum majorana50,827964,33820260604-patchedfull
Origanum vulgare44,141915,36220260604-patchedfull
Perilla citriodora23,675863,73720260604-patchedfull
Perilla frutescens38,941896,31820260604-patchedfull
Perilla frutescens var. hirtella23,675863,73720260604-patchedfull
Perovskia atriplicifolia35,771903,86420260604-patchedfull
Petraeovitex bambusetorium42,920934,02320260604-patchedfull
Phlomis fruticosa58,479934,75020260604-patchedfull
Phlomoides rotata51,686976,58120260604-patchedfull
Plectranthus barbatus46,489935,03120260604-patchedfull
Pogostemon cablin132,4079919,60320260604-patchedfull
Premna microphylla45,762914,23620260604-patchedfull
Prostanthera lasianthos46,730924,07720260604-patchedfull
Prunella vulgaris45,001915,47820260604-patchedfull
Rotheca myricoides59,312935,20120260604-patchedfull
Salvia bowleyana44,032904,36420260604-patchedfull
Salvia divinorum38,973925,68620260604-patchedfull
Salvia hispanica46,507907,39920260604-patchedfull
Salvia miltiorrhiza29,236894,40920260604-patchedfull
Salvia officinalis36,084923,76920260604-patchedfull
Salvia rosmarinus51,386916,91220260604-patchedfull
Salvia sclarea17,186892,16620260604-patchedfull
Salvia splendens79,2499110,99920260604-patchedfull
Schizonepeta tenuifolia27,971863,87820260604-patchedfull
Scutellaria baicalensis30,090914,52020260604-patchedfull
Scutellaria barbata34,557924,06520260604-patchedfull
Stachys officinalis70,031934,34420260604-patchedfull
Stenogyne calaminthoides77,090959,96920260604-patchedfull
Tectona grandis35,551945,91320260604-patchedfull
Teucrium canadense38,989903,99120260604-patchedfull
Teucrium chamaedrys128,0139719,01220260604-patchedfull
Teucrium marum73,4669010,38720260604-patchedfull
Thymus quinquecostatus29,676904,70420260604-patchedfull
Thymus vulgaris52,878954,58520260604-patchedfull
Vitex agnus-castus42,689924,47220260604-patchedfull
Westringia fruticosa40,051913,75020260604-patchedfull

Known limitations

Interpretation boundaries

Candidate function

Computational enzyme-family candidates and genome-structure support, not validated biochemical function.

Synteny

Local 100-kb clustering proxy, not full cross-species synteny.

Coverage

Only 80 of 808 database species currently expose public candidate identifiers. Missing profiles are not interpreted as pathway absence.

Public sequence boundary

Candidate source fields may include protein length, but public protein sequence records are not released by the current LamiOmicsDB interface.